yydI [2020-03-05 09:50:58]

[SW|ABC transporter], ATP-binding protein, export of the mature [protein|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|YydF] epipeptide

locus: BSU_40150

pI: 8.39

mw: 23.74 kDa

protein length: 209 aa

gene length: 630 bp

function: control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|LiaS] activity

product: [SW|ABC transporter], ATP-binding protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,124,963 4,125,592

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• [SW|ABC transporter domain] (aa 1-207) (according to UniProt)

Structure

• [PDB|4HLU] (aa 7- 175, Thermotoga maritima EcfA, 32% identity) [pubmed|23359690]

[SW|Localization]

• membrane associated (via [protein|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|YydJ]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|yydF]-[gene|25FC87037222084ABE318813959101016FEB49DF|yydG]-[gene|E280A5994F4D9D93DA4B698E79C86360DBEE5396|yydH]-[gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|yydI]-[gene|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|yydJ]
  description: [Pubmed|17921301]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17921301], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|17921301], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]

  regulation

  • activated after transition to stationary phase([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]) [Pubmed|17921301]

Biological materials

Mutant

• MGNA-B808 (yydI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1807 NBRP B. subtilis, Japan]
• BKE40150 ([gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|yydI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATACATACTCCTAGGAA, downstream forward: _UP4_TAATAAAACAGTGGGGGCTT
• BKK40150 ([gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|yydI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATACATACTCCTAGGAA, downstream forward: _UP4_TAATAAAACAGTGGGGGCTT

References

Reviews

• 23106164

Original publications

• 10092453,23199363,17921301,15743949,17921301,21815947,28644475,23359690